PCR Sequencing Protocols
Ralph Rapley
A superb, comprehensive collection of the most reliable protocols for PCR sequencing-all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing.
Categorie:
Anno:
1996
Edizione:
1st
Casa editrice:
Humana Press
Lingua:
english
Pagine:
218
ISBN 10:
0896033449
ISBN 13:
9780896033443
Collana:
Methods in Molecular Biology 065
File:
PDF, 14.22 MB
IPFS:
,
english, 1996
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