RT-PCR Protocols
Nicola King, Joe O'Connell
A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
Categorie:
Anno:
2002
Edizione:
1st
Casa editrice:
Humana Press
Lingua:
english
Pagine:
394
ISBN 10:
0896038750
ISBN 13:
9780896038752
Collana:
Methods in Molecular Biology
File:
PDF, 4.39 MB
IPFS:
,
english, 2002