Plant Virology Protocols
Foster G. D. (Ed), Taylor S. (Ed)
A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.
Volume:
Volume 81
Anno:
1998
Lingua:
english
Pagine:
571
File:
RAR, 29.19 MB
IPFS:
,
english, 1998
Amazon 
Barnes & Noble 
Bookshop.org 
Converti i file
Più risultati di ricerca
Altri vantaggi 
Termini più frequenti
Liste di libri correlati
